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Visualizing and perturbing the embryonic cardiovascular system with light
Author(s) -
Jenkins Michael W.,
Wang Yves T.,
Peterson Lindsy M.,
Gu Shi,
Karunamuni Ganga H.,
Ma Pei,
Rollins Andrew M.,
Watanabe Michiko
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.313.1
Subject(s) - embryonic heart , optical coherence tomography , optical mapping , coherence (philosophical gambling strategy) , cardiac function curve , heart development , neuroscience , biomedical engineering , electrical conduction system of the heart , anatomy , computer science , medicine , physics , embryonic stem cell , cardiology , biology , optics , heart failure , electrocardiography , biochemistry , quantum mechanics , gene
Biophysical forces impinging on cardiac cells and tissues are important signals needed for proper development of the cardiovascular system. Unfortunately, the role of these signals are not fully identified nor well understood due to a lack of proper tools to quantitatively assess the causal relationship between mechanical/electrical forces and phenotype. We developed a suite of optical imaging systems (optical coherence tomography, optical coherence microscopy and optical mapping) capable of quantifying morphologic features (e.g. endocardial cushion size), cardiac function (e.g. blood flow, conduction) and biophysical forces (e.g. shear stress, etc.) in the early stages of heart development. Also, we are developing a new method (optical pacing) to precisely perturb cardiac dynamics. These set of tools will enable us to investigate how altered function in the earliest stages of heart development potentially lead to congenital heart defects. Research Support: NIH grants HL083048, HL095717 HL, HL115373.