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Imaging the cellular dynamics and signals that pattern embryos
Author(s) -
Fraser Scott E.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.312.1
Subject(s) - microscopy , microscope , light sheet fluorescence microscopy , live cell imaging , two photon excitation microscopy , organogenesis , resolution (logic) , intravital microscopy , temporal resolution , computer science , biology , artificial intelligence , cell , optics , physics , biochemistry , genetics , microbiology and biotechnology , scanning confocal electron microscopy , gene , in vivo , fluorescence
Advances in cell biology, genomics and proteomics offer unprecedented knowledge of the constituents within cells and the means by which the cells can interact. Advanced imaging techniques provide a powerful means of integrating this growing data set into a mechanistic understanding of the cellular mechanisms that underlie embryogenesis and organogenesis, by studying the events in the intact system. These powerful techniques are challenged by major tradeoffs between spatial resolution, temporal resolution, and the limited photon budget. This combination of requirements is challenging, but necessary: intravital imaging can only generate accurate data on cell lineages and cell migration if it can capture the three dimensional image of the entire embryo or tissue before any of the cells can move half of the distance separating neighboring cells. We are attempting to advance these tradeoffs by constructing faster and more efficient microscopes that maintain subcellular resolution. Our new microscope combines the deep penetration of two‐photon microscopy and the speed of light sheet microscopy (often termed SPIM) to generate images with more than ten‐fold improved imaging speed and sensitivity. This two‐photon SPIM is far less subject to light scattering, permitting subcellular resolution to be maintained far better than conventional light sheet microscopes.