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Carbohydrate co‐ingestion with protein delays dietary protein digestion and absorption but does not modulate postprandial muscle protein accretion
Author(s) -
Gorissen Stefan H,
Burd Nicholas A,
Hamer Henrike M,
Gijsen Annemie P,
Loon Luc JC
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.249.6
Subject(s) - postprandial , ingestion , carbohydrate , medicine , endocrinology , phenylalanine , digestion (alchemy) , chemistry , biology , biochemistry , amino acid , insulin , chromatography
We determined the impact of carbohydrate co‐ingestion with protein on dietary protein digestion and absorption kinetics and postprandial muscle protein accretion in healthy men. Twenty‐four young (age: 21±1 y, BMI: 21.8±0.5 kg/m 2 ) and 24 older (age: 75±1 y, BMI: 25.4±0.6 kg/m 2 ) men received a primed continuous L‐[ring‐ 2 H 5 ]‐phenylalanine infusion and ingested 20 g L‐[1‐ 13 C]‐ phenylalanine‐labeled protein with (PRO+CHO) or without (PRO) 60 g carbohydrate. Biopsies were collected from the vastus lateralis . Carbohydrate co‐ingestion delayed the rise in exogenous phenylalanine appearance rate (P<0.05). Dietary protein‐derived phenylalanine availability over the postprandial period was lower in the old (60±2%) when compared with the young (72±2%; P<0.05), with no differences between the PRO and PRO+CHO condition (P=0.42). Carbohydrate co‐ingestion did not modulate postprandial muscle protein synthesis rates (0.035±0.003 vs 0.043±0.004 and 0.033±0.002 vs 0.035±0.003 %/h following PRO vs PRO+CHO in the young and old, respectively). In accordance, no differences in muscle protein‐bound L‐[1‐ 13 C]‐phenylalanine enrichments were observed between groups (0.020±0.003 vs 0.018±0.003 and 0.017±0.003 vs 0.022±0.004 MPE following PRO vs PRO+CHO in the young and old, respectively). We conclude that carbohydrate co‐ingestion does not modulate postprandial muscle protein accretion in young or older men.