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Early life exposure to soy isoflavones in combination with an adequate but not supplemental level of folic acid improves bone development of CD‐1 mice by suppressing expression of neuropeptide Y
Author(s) -
Kaludjerovic Jovana,
Ward Wendy E
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.247.7
Subject(s) - endocrinology , medicine , bone mineral , offspring , osteoporosis , femur , cortical bone , bone density , lactation , bone resorption , pregnancy , biology , anatomy , surgery , genetics
Early life exposure to soy isoflavones (ISO) improves bone health of female mice at adulthood. This benefit may be mediated by methylation of CpG sites that regulate transcription of genes involved in bone formation. The objective was to examine if early life exposure to ISO and supplemental folic acid (FA), a methyl donor, induces permanent changes in bone‐specific gene expression that result in stronger bones at adulthood in female CD‐1 mice. Mice were randomized to adequate (2 mg/kg diet) or supplemental (8 mg/kg diet) FA during pregnancy and lactation. Offspring received corn oil or ISO (7 mg/kg body weight/day) from day 1 to 10 of life and adequate FA diet until age 4 months. Contrary to our hypothesis, exposure to ISO + supplemental FA had a modest effect on lumbar spine (higher bone mineral density (BMD)) with no effect on femurs. This may be due to higher expression of bone resorption genes, i.e. beta‐catenin and parathyroid hormone receptor 1. In contrast, females exposed to adequate FA + ISO had higher BMD, improved trabecular connectivity and greater strength at the femur and lumbar spine that may be explained by lower expression of neuropeptide Y – a neurotransmitter that is inversely associated with bone formation. Early life exposure to ISO has contrasting effects on adult bone health, depending on the level of FA in the diet. Benefits to BMD, bone structure as well as bone strength were only observed with adequate FA. Grant Funding Source : Canadian Institutes of Health Research (funding number 89941) and a Frederick Banting and Charles Best Canada Graduate Scholarship