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Neonatal dietary calcium alters mesenchymal stem cell differentiation potential in pigs
Author(s) -
Li Yihang,
Seabolt Brynn,
Stahl Chad
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.244.5
Subject(s) - adipogenesis , mesenchymal stem cell , osteocalcin , runx2 , population , andrology , calcium , biology , stem cell , in vitro , endocrinology , chemistry , medicine , alkaline phosphatase , microbiology and biotechnology , biochemistry , osteoblast , enzyme , environmental health
Neonatal Ca nutrition affects bone development and mesenchymal stem cell (MSC) activity; however, its effect on MSC differentiation potential has not been fully characterized. MSC were isolated from neonatal pigs fed either a Ca deficient, adequate, or excessive diet. After in vitro adipogenic induction, PPARG and AP2 expression was up‐regulated in MSC from Ca deficient (P<0.1) and Ca excess (P<0.05) groups. Under osteogenic induction, osteocalcin (OC) gene expression was depressed in MSC from Ca deficient pigs. To further evaluate the impact of Ca status on MSC activity, 20 individual colonies were obtained from each pig. From these colonies, we selected the most and least osteogenic (O+/O−) and the most and least adipogenic (A+/A−) clones based on functional staining. During osteogenesis, increased (P < 0.1) gene expression of Runx2 and OC was seen among O+ colonies. Increased expression of OC was seen among Ca adequate (P<0.01) and excess (P=0.07) colonies compared with the Ca deficient colonies. During adipogenesis, A+ colonies had greater expression (P < 0.1) of AP2 and LPL. No interaction between osteogenic or adipogenic capability and Ca status was seen. This suggests that the differences in adipocytic and osteogenic differentiation of MSC based on the Ca status of the pig is due to alterations in the number of highly osteogenic and adipogenic MSC in the heterogeneous population. Grant Funding Source : NC Agricultural Research Service