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Supplementation with Zinc Affects Serum MicroRNA (miRNA) Levels in Early Pubertal Females
Author(s) -
Grider Arthur,
Lewis Richard,
Laing Emma,
Wickwire Kathie
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.234.2
Subject(s) - zinc , microrna , medicine , placebo , endocrinology , physiology , immunology , biology , gene , chemistry , genetics , pathology , organic chemistry , alternative medicine
The purpose of this research was to determine whether supplementation with oral zinc near the RDA for 4 wks would alter serum miRNA levels among females in the early stages of puberty. Black and white females, 9 to 13 years of age, were given either 9 mg elemental zinc (Zn; n=75) or placebo (PL; n=71) for 4 wks. Sera were collected from subjects at baseline and 4 wks, and analyzed for zinc by atomic absorption. There were no differences in baseline characteristics between Zn and PL groups. Blacks had higher serum zinc concentrations at baseline and 4 wks. Following supplementation serum Zn increased more in the Zn group vs PL (p < 0.05). There was no difference in the serum zinc response to zinc supplementation between blacks and whites. Sera from subjects that were most responsive to zinc supplementation (n = 5) or exhibited the least change in serum Zn (n = 5) were pooled. Serum exosomes from each sample were purified using a magnetic‐bead based isolation procedure. The RNAs were analyzed using the Affymetrix GeneChip miRNA 3.0 microarrays. Thirteen and 12 miRNAs changed at least 1.75‐fold in response to zinc supplementation in blacks and whites, respectively; however, there was no overlap in miRNAs species between blacks and whites. These miRNAs may ultimately serve as serum biomarkers for assessing zinc status in young girls. However, zinc biomarkers associated with race may need to be considered.