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Key markers associated with intestinal adaptation in pediatric short bowel syndrome
Author(s) -
Naberhuis Jane Kelly,
Tappenden Kelly Anne
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.225.8
Subject(s) - glucagon like peptide 2 , crypt , short bowel syndrome , parenteral nutrition , adaptation (eye) , enteral administration , biology , intestinal resection , gastroenterology , medicine , intestinal failure , resection , surgery , peptide , biochemistry , neuroscience
Administration of partial enteral nutrition (PEN) and trophic peptides, such as glucagon‐like peptide‐2 (GLP‐2), are emerging strategies for stimulating structural and functional aspects of intestinal adaptation in individuals with intestinal failure. To investigate the temporal sequence of events associated with PEN and/or GLP‐2‐mediated intestinal adaptation following massive small bowel resection, neonatal piglets underwent an 80% jejunoileal resection (n=72) and were randomized to 1 of 4 treatment groups using a 2×2 factorial model with total parenteral nutrition (TPN) or TPN+PEN and placebo or GLP‐2. Piglets were euthanized 4h, 48h or 7d after surgery and 93 variables of adaptation were analyzed by principal component analysis (PCA). Maximal variation was accounted for by small intestinal proliferation, apoptosis and crypt depth when all time points were pooled. The main factors accounting for variance shifted from crypt depth at 4h, to small intestinal nutrient transport and cellular proliferation at 48h. By d7, mucosal architecture, epithelial proliferation and nutrient transport accounted for maximal variation. These results indicate that structural markers of adaptations precede those of functional adaptation; however, crypt depth remains a strong indicator, regardless of time. Grant Funding Source : Internally funded