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Quercetin encapsulated nanocarriers: effects on breast cancer cell growth, apoptosis, and uptake in vitro and bioavailability in vivo
Author(s) -
Sun Ming,
Nie Shufang,
Pan Xuan,
Fan Zhaoyang,
Wang Shu
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.224.3
Subject(s) - quercetin , bioavailability , in vivo , mcf 7 , nanocarriers , propidium iodide , viability assay , apoptosis , chemistry , cancer cell , pharmacology , breast cancer , annexin , flavonoid , cancer , cancer research , biochemistry , medicine , biology , programmed cell death , antioxidant , drug , microbiology and biotechnology , human breast
Background Quercetin, a natural flavonoid, has a potential against many breast cancer cells, but its low solubility and bioavailability in the body make administering it in therapeutic dose unrealistic. We have successfully synthesized quercetin encapsulated nanocarriers (NanoQ). Our hypothesis is that NanoQ can enhance quercetin stability and solubility, increase quercetin bioavailability in vitro and in vivo, decrease the viability of breast cancer cells, and induce their apoptosis. Methods The stability, solubility and cellular uptake of quercetin in MCF7 and MDA‐MB‐231 breast cancer cells were measured using a high performance liquid chromatography system. The viability and apoptosis of breast cancer cells were measured using a 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay and Annexin‐V/propidium iodide assay, respectively. The pharmacokinetics of NanoQ was measured in SD rats. Results NanoQ were about 30 nm in diameter. Nanoencapsulation significantly increased the stability and solubility of quercetin, and increased quercetin cellular content 3.9 times and 9.3 times in MCF7 and MDA‐MB‐231 cells, respectively. NanoQ also significantly lowered the viability of both breast cancer cells and induced their apoptosis as compared to free quercetin at the same concentrations. Conclusion NanoQ is a promising approach for the prevention and treatment of breast cancer. Grant Funding Source : TTU research incentive funding