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Analysis of RNA polymerase II transcription initiation and elongation complexes
Author(s) -
Buratowski Stephen
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.210.3
Subject(s) - rna polymerase ii , transcription factor ii f , transcription factor ii d , rna polymerase ii holoenzyme , transcription factor ii e , rna polymerase i , polymerase , transcription (linguistics) , general transcription factor , chromatin , rna polymerase , rna , biology , computational biology , microbiology and biotechnology , genetics , gene , gene expression , promoter , linguistics , philosophy
Our lab uses a variety of approaches to try to understand how RNA polymerase II initiates, synthesizes, and terminates transcription. We are also very interested in how transcription is coupled to the processes of RNA processing and chromatin modification. The yeast Saccharomyces cerevisiae is an outstanding model system, allowing genetic, genomic, molecular, and biochemical approaches to be effectively combined. In collaboration with Dr. Jarrod Marto (HMS/DFCI), we are using quantitative proteomics experiments to analyze the proteins associated with RNA polymerase II complexes assembled on naked and chromatinized templates. We also continue our work on how the phosphorylation patterns on the C‐terminal domain (CTD) of RNA polymerase II subunit Rpb1 are used to recruit important RNA processing, chromatin‐modifying, and termination factors at specific points of the transcription cycle. I will be presenting some of our recent results describing the communication between RNA polymerase II, its associated factors, and the chromatin template.