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Spatiotemporal Control of Endocytosis by Phosphatidylinositol 3,4‐Bisphosphate
Author(s) -
Haucke Volker,
Posor York
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.202.1
Subject(s) - endocytosis , endocytic cycle , phosphatidylinositol , microbiology and biotechnology , pi , clathrin , phosphatidylinositol 4,5 bisphosphate , receptor mediated endocytosis , function (biology) , biology , pi3k/akt/mtor pathway , kinase , chemistry , cell , signal transduction , biochemistry
Phosphoinositides (PIs) serve crucial roles in cell physiology ranging from cell signalling to intracellular membrane traffic. While the function of many PIs has been studied in detail little is known about the role of phosphatidylinositol‐3,4‐bisphosphate [PI(3,4)P2]. Here, we show that formation of PI(3,4)P2 by class II phosphatidylinositol 3‐kinase C2α (PI3K C2α) spatiotemporally controls clathrin‐mediated endocytosis. Depletion of PI(3,4)P2 or PI3K C2α impairs the maturation of clathrin‐coated pits at a late stage preceding fission. Timed formation of PI(3,4)P2 by PI3K C2α is required for the selective enrichment of the BAR domain protein SNX9 at late‐stage endocytic intermediates, in accordance with mathematical modelling. These findings provide a mechanistic framework for the role of PI(3,4)P2 in endocytosis and unravel a novel discrete function of PI(3,4)P2 in a central cell physiological process.