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Neutrophil Microparticles are heterogenous in content and function
Author(s) -
Perretti Mauro,
Dalli Jesmond,
Norling Lucy V,
Melendez Trini Montero,
Hinds Charles
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.138.5
Subject(s) - microparticle , microbiology and biotechnology , chemistry , immunology , stimulation , inflammation , reactive oxygen species , biology , neuroscience , astrobiology
Altered plasma neutrophil‐derived microparticle levels have recently been implicated in a number of vascular and inflammatory diseases, yet our understanding of their actions is very limited. Stimulation of neutrophils, either in suspension or adherent to an endothelial monolayer, led to the production of microparticles containing >;600 distinct proteins with 247 being shared by the two subsets. Assessing the expression profile of a number of validated proteins in three types of human samples (healthy volunteer and septic plasma along with blister‐derived exudates) confirmed that specific protein abundance in the microparticles might reflect the activation status/mode of the neutrophil. The two microparticle subsets responded to external stimuli showing that ‐ when appropriately stimulated ‐ they release reactive oxygen species, synthesise leukotriene B4 and chemotax. These findings demonstrate that neutrophil‐derived microparticles are heterogenous and can deliver packaged information propagating the parent cell's activation status, potentially playing a novel and fundamental role both under homeostatic and disease conditions.