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Neutrophil‐Derived Microparticles as Novel Effectors in Joint Disease
Author(s) -
Headland Sarah Emily,
Dell'Accio Francesco,
Perretti Mauro
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.137.6
Subject(s) - cartilage , flow cytometry , context (archaeology) , synovial fluid , inflammation , chemistry , microbiology and biotechnology , rheumatoid arthritis , immunology , articular cartilage , arthritis , synovial membrane , synovial joint , pathology , medicine , biology , osteoarthritis , anatomy , paleontology , alternative medicine
Microparticles (MPs) are small membrane‐bound vesicles released by cells upon activation. Numerous reports have detected MPs in the synovial fluid of Rheumatoid Arthritis (RA) patients. Whilst Tcell‐ derived MPs activate synoviocytes, recent reports have shed light on the possible anti‐inflammatory phenotype of neutrophilderived MPs, challenging the linear model of inflammation. The aim of the study is to unveil the role of neutrophil‐derived MPs in RA, particularly in the context of cartilage biology. Microparticles generated from human neutrophils were characterised by flow cytometry and tested for chondroprotective efficacy in a C28/I2 micromass model of cartilage turnover. These experiments revealed protective actions. Investigation in whole cartilage explants indicated chondroprotection (demonstrated by safranin O staining) as studied over a seven‐day period. Microparticle entry into cartilage was also investigated using confocal microscopy. In conclusion, we report novel biological functions of neutrophil MPs with potential relevance to cartilage biology and arthritis. Supported by Oliver Bird Studentship Scheme QMUL/KCL