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Resolvin D1 Receptor Activation Counter‐regulates H1 histamine receptors in human and rat conjunctival goblet cells
Author(s) -
Hodges Robin R.,
Li Dayu,
Carozza Richard B.,
Jiao Jianwei,
Shatos Marie A.,
Chiang Nan,
Serhan Charles N.,
Dartt Darlene A.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.132.6
Subject(s) - histamine , histamine h1 receptor , chinese hamster ovary cell , histamine receptor , receptor , g protein coupled receptor , agonist , histamine h4 receptor , chemistry , endocrinology , microbiology and biotechnology , transfection , protein kinase c , medicine , histamine h2 receptor , pharmacology , biology , kinase , biochemistry , gene , antagonist
The purpose of this study was to determine the interactions between H1 histamine receptor (H1R) and GPR32, a GPCR receptor for the proresolution mediator, resolvin D1 (RvD1). Rat conjunctival goblet cells were grown in culture. Chinese hamster ovary (CHO) cells were transfected with human H1R and GPR32 receptor. Intracellular [Ca 2+ ] ([Ca 2+ ] i ) was measured in cells loaded with the calcium indicator dye fura 2. In rat goblet cells, the H1R agonist histamine dimaleate significantly increased [Ca 2+ ] i by 327.2 ± 71.5 nM. Exposure to RvD1 significantly inhibited this response to 92.6 ± 21.5 nM. The protein kinase C inhibitor Ro‐ 317549 and an inhibitory peptide of β adrenergic receptor kinase 1 (βARK1) reversed the inhibition of RvD1 on the histamine dimaleate stimulated increase in [Ca 2+ ] i to 197.2 ± 46.3 and 352.7 ± 89.6 nM, respectively. Similar results were obtained in CHO cells transfected with human H1R and human GPR32 receptor. We conclude that RvD1 uses PKC and βARK1 to counter‐regulate the H1R and inhibit its actions.