Premium
Relationship Between In Vitro Absorption of Chondroitin Sulfate (CS) and Its Molecular Weight
Author(s) -
Surapaneni Lahari,
HaleyZitlin Vivian,
Bodine Ashby,
Jiang Xiuping,
Brooks James
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.125.4
Subject(s) - chondroitin sulfate , chemistry , absorption (acoustics) , sulfate , molecular mass , chromatography , in vitro , biochemistry , glycosaminoglycan , organic chemistry , enzyme , materials science , composite material
Objective The purpose of this study was to investigate if the molecular weight of chondroitin sulfate (CS) has an impact on its absorption in vitro , in a Caco‐2 cell model. Methods Caco‐2 cells in 24 well plates were incubated for 2 hours with eight CS samples of differing molecular weights (7 kDa – 35 kDa). The amount of CS transported into the basolateral side of the Caco‐2 monolayer was quantitatively determined to calculate the permeability coefficients (P app ). Results Of the 8 samples evaluated, four samples had a P app value of 15 × 10 −6 cm sec −1 or higher indicating moderate to high absorption. Two of the four samples with higher P app values were high molecular weight compounds (~ 35 kDa). Conclusion Within the molecular weight range used in this experiment, the absorption of CS samples did not have a correlation with their molecular weights but, interestingly, correlation was observed between the absorption and percentage of 6‐sulfated disaccharide in the CS samples. Grant Funding Source : Pharmavite, Inc.