Premium
DHA‐enriched fish oil modulates B cell function and modifies clustering of lipid microdomains
Author(s) -
Gurzell Eric Anthony,
Teague Heather,
Harris Mitchel,
Clinthorne Jonathan,
Shaikh Saame Raza,
Fenton Jenifer
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.123.6
Subject(s) - fish oil , population , b cell , t cell , biology , in vivo , cd40 , ex vivo , interleukin 4 , chemistry , immune system , immunology , microbiology and biotechnology , biochemistry , in vitro , cytotoxic t cell , antibody , fish <actinopterygii> , medicine , environmental health , fishery
Fish oil has significant immunomodulatory effects and is primarily associated with suppression of T lymphocyte and dendritic cell function. The purpose of this study was to investigate the changes that DHA‐enriched fish oil (DFO) feeding elicits on B cells, an understudied, yet important cell population with respect to inflammatory pathologies. Utilizing confocal microscopy and flow cytometric analyses of purified splenic B cells, we assessed the effects of DFO on B cell activation in 129Sv mice (n=10/treatment). DFO feeding altered the lipid composition of splenic B cells and diminished GM1 microdomain clustering in the membrane. The clusters on B cells from mice fed the DFO diet appear 0.275 μm larger compared to control fed mice. In vivo, DFO increased lymphoid tissue B cell populations and surface markers of activation compared to controls. Ex vivo, LPS‐stimulated B cells isolated from DFO fed mice expressed more CD40 and produced more IL‐6 (50% increase) and TNF‐α (40% increase) than those from control mice, however OVA‐FITC antigen uptake was similar. DFO fed mice had significantly higher plasma IL‐5, IL‐13, and IL‐9 (Th2‐biasing cytokines) and cecal IgA compared to control. These results support the hypothesis and an emerging concept that fish oil enhances B cell function in vivo. These data are consistent with observations in C57Bl/6 mice, suggesting dietary fish oil enhances humoral immunity. Grant Funding Source : NIH R03CA162427 (to J.I.F.) and NIH R15AT006122 (to S.R.S.)