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Dietary supplementation with white button mushroom augments the protective immune response to Salmonella vaccine in mice
Author(s) -
Wang Junpeng,
Niu Xiuli,
Du Xiaogang,
Smith Donald,
Meydani Simin Nikbin,
Wu Dayong
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.123.2
Subject(s) - immune system , salmonella , immunization , vaccination , spleen , biology , microbiology and biotechnology , pathogen , immunology , antibody , bacteria , genetics
We previously showed that dietary white button mushrooms (WBM) enhance natural killer cell activity and in vitro WBM promotes maturation and function of dendritic cells (DC). The current study investigated if WBM consumption could enhance pathogen‐specific immune response. C57BL/6 mice were fed diets containing 0, 2, or 5% WBM for 4 wk before oral vaccination with live attenuated Salmonella typhimurium . Four wk after immunization, mice were orally infected with S. typhimurium SL1344. Results showed that immunization increased animal survival while WBM alone had no effect; however, among immunized mice, 2% WBM group had a higher survival rate than other groups. We then fed mice 2% WBM to determine the underlying mechanism. We found that Salmonella‐ specific blood IgG and fecal IgA levels were higher in WBM‐fed mice than the control mice. WBM‐fed mice also had higher levels of IgG2a and unchanged IgG1 leading to a higher IgG2a/IgG1 ratio, which indicates an enhanced Th1 response. We further found that at 4 wk post‐immunization, WBM‐fed mice had more DC in spleen, and these DC expressed higher levels of the activation markers CD40 and MHC‐II. Together, these results suggest that WBM may improve the adaptive immune response to Salmonella , a food‐borne pathogen, at least in part through activating DC function. Supported by the Mushroom Council and mushroom industry funding partners, and the USDA contract #58–1950‐0–014.