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In vitro antibacterial activity of the leucocyte and platelet‐rich fibrin clot exudate (L‐PRF)
Author(s) -
Bozelli Ana Flavia,
Menezes Reiff Rodrigo Bezerra,
Semeghini Dalmyr Osmar,
Corbi Pedro Paulo,
Lustri Wilton Rogério,
Amaral André Capaldo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1217.32
Subject(s) - exudate , fibrin , platelet , pseudomonas aeruginosa , in vitro , microbiology and biotechnology , chemistry , antibacterial activity , pharmacology , medicine , bacteria , biology , immunology , biochemistry , pathology , genetics
The leucocyte and platelet‐rich fibrin (L‐PRF) is a potential tool to investigate the effects attributed to platelet concentrates. Although able to stimulate tissue repair, there are no reports regarding its antibacterial activity. To evaluate the action of the exudate extracted from the L‐PRF in vitro , blood samples of 10 male volunteers (ages 18–24) were centrifuged in clot activator tubes. The clots were shaken vigorously after 1 and 6 hours of activation for the extraction of exudate. Platelet (PLA), leucocyte (LEU) and erythrocyte (ERY) counting was performed in whole blood, platelet‐poor serum (PPS) and exudate. The antibacterial activity was investigated by disk diffusion technique in strains of Escherichia coli (ATCC 25922) , Pseudomonas aeruginosa (27853) and Staphylococcus aureus (25923), using specific antibiotics as positive controls. Cell counting in whole blood yielded a value of 6 ± 1 × 10 3 for LEU, 226 ± 28 × 10 3 for PLA and 5 ± 0.3×10 6 for ERY; 1‐hour PPS: 0.1 ± 0.1 to LEU and 0.5 ± 0.8 for PLA; 6‐hour PPS: 0.1 ± 0.2 to LEU and 0.4 ± 0.7 for PLA; exudate 1 hour: 5 ± 4 to LEU and 82 ± 56 for PLA; exudate 6 hours: 8 ± 7 to LEU and 211 ± 240 for PLA. The values of the ERY in the exudate and PPS were negligible. The exudates and PPS samples did not generate an inhibition zone and the positive controls exhibited halos with specific inhibition patterns. The L‐PRF exudate showed no antibacterial activity. Financial Support : CNPq Brazil