Premium
Extracellular matrix properties regulates the phenotypic and genetic features of human epidermal keratinocytes
Author(s) -
KIM MINA,
Shin Dong Wook,
Nho Minsoo,
Shin Jennifer Hyunjong
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1217.25
Subject(s) - extracellular matrix , vimentin , mesenchymal stem cell , keratinocyte , microbiology and biotechnology , fibroblast , phenotype , chemistry , epithelial–mesenchymal transition , wound healing , downregulation and upregulation , in vitro , biology , immunology , immunohistochemistry , biochemistry , gene
Keratinocytes, like most other cells, are sensitive to their physicochemical conditions of their microenvironment, being able to sense and respond to different rigidity as well as ECM (extracellular matrix) protein type. In this study, we investigated that how ECM protein type and rigidity regulate keratinocyte. To investigate the effects of ECM protein, normal human epidermal keratinocytes (NHEKs) were cultured on three different ECM (LN, FN, COL) coated glass for 13days. Keratinocytes undergo noticeable changes in the morphology and exhibit mesenchymal features typically observed in fibroblast on FN or COL coated glass. Real time PCR results showed that NHEKs on FN or COL coated glass showed mesenchymal features indicated by relative up‐and down‐regulation of vimentin and E‐cadherin, respectively. To investigate the applicability on therapeutic, NHEKs were cultured on three different ECM coated PDMS, biocompatible material. Keratinocyte on PDMS showed more mesenchymal features compared to ones cultured on glass. Phenotypic and genetic changes were observed in FN or COL coated PDMS cultured cells compared to LN coating. Considering the transition of keratinocyte from epithelial to mesenchymal feature occur during re‐epithelialization of wound healing, our results suggest that silicon including FN or COL plays a central role in wound repair and skin therapy.