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Modulation of BK Ca channel activities by calcium sensing receptor in rat bronchopulmonary sensory neurons
Author(s) -
Vysotskaya Zhanna,
Gilbert Carolyn,
Gu Qihai
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1214.12
Subject(s) - iberiotoxin , bk channel , chemistry , calcium sensing receptor , patch clamp , agonist , antagonist , receptor , neuroscience , medicine , endocrinology , potassium channel , biophysics , calcium , biology , calcium metabolism , biochemistry , organic chemistry
Large‐conductance Ca 2+ ‐activated potassium (BK Ca ) channels play an important role in shaping the neuronal excitability, but little is known about their expression and properties in bronchopulmonary sensory neurons. We have demonstrated recently the functional expression of extracellular calcium‐sensing receptors (CaSR) in rat vagal bronchopulmonary afferents. The aim of this study was to characterize the BK Ca currents in isolated rat bronchopulmonary sensory neurons and to investigate the possible modulating effect of CaSR on these currents. Whole‐cell perforated patch‐clamp recordings were carried out and BK Ca currents were isolated from total K + currents with paxilline and iberiotoxin, two selective BK Ca channel blockers. Our results showed that there was a large variability in BK Ca current densities among individual neurons, and paxilline seems more potent in blocking these currents comparing to iberiotoxin. Pretreatment with CaSR antagonist NPS 2143 dramatically inhibited BK Ca currents, whereas activation of CaSR with its agonist NPS R‐568 potentiated BK Ca currents in these neurons. In summary, our data suggested that CaSR plays an integrative role in bronchopulmonary afferent signaling, at least partially through the regulation of BK Ca channel activities. Funded by AHA grant and MUSM startup funds.