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Carbon Nanotube, Nanosilver and Nanoclay Protein Corona Composition in Cell Culture Media
Author(s) -
Lai Xianyin,
Shannahan Jonathan H.,
Chen Ran,
Ke PuChun,
Mitra Somenath,
Brown Jared M.,
Witzmann Frank A.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1212.8
Subject(s) - carbon nanotube , nanomaterials , nanoparticle , nanotoxicology , silver nanoparticle , chemistry , surface modification , composition (language) , in vitro , protein adsorption , chemical engineering , corona (planetary geology) , nanotechnology , materials science , adsorption , biophysics , biochemistry , organic chemistry , linguistics , philosophy , physics , astrobiology , venus , engineering , biology
During in vitro assessment of nanoparticle (NP) exposure in cell culture systems, NPs adsorb serum proteins in media to form a protein corona (PC). The composition of this PC may determine the nature of cellular interaction and biological effect. Using a label‐free quantitative mass spectrometry‐based proteomics approach, IdentiQuantXL, we examined the composition of the PC for various well‐characterized nanomaterials, including unmodified and carboxylated single‐(SWCNT) and multi‐walled carbon nanotubes (MWCNT), PVP‐coated MWCNT, nanoclay, and PVP‐and citrate‐coated silver nanoparticles. All nanotubes were found to associate with a common subset of 14 proteins and SWCNT‐COOH and MWCNT‐COOH were found to associate with the greatest number of proteins (181 and 133) compared to the unmodified nanotubes (<100). PVP or citrate capping did not significantly influence the protein types found in the AgNP PC, while comparison of AgNP and nanotube coronas revealed significantly different protein constituents and complexity. In conclusion, we observed differential protein corona composition based on functionalization, purity, and shape, which may influence the unique biological effects of these NPs in vitro.