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Characterizing the co‐occurrence between mitochondrial KATP channel and uncoupling proteins in type 2 diabetes
Author(s) -
Brito Alfonso,
Peltekian Lila,
Mandala Sridhar,
Gonzalez Nelson,
Lopez Vanessa,
Yamazaki Katrina
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1209.23
Subject(s) - cardioprotection , type 2 diabetes , medicine , mitochondrion , endocrinology , uncoupling protein , diabetes mellitus , biology , obesity , ischemia , microbiology and biotechnology , brown adipose tissue
Patients with type 2 diabetes (T2D) have a heightened risk of developing heart failure due to mitochondrial abnormalities and sporadic cardiac energy metabolism. The main objective of this project is to determine mitochondrial KATP channel dysfunction and UCP‐2 expression relative to each other utilizing an in vivo rat model of T2D. Administration of epicatehin, a cocoa flavanol, was tested for cardioprotection in T2D. Results demonstrated that diabetic animals had significant increases in body weight and blood glucose levels, while EPI treatment had changes similar to sham animals. Western analysis show an overall decrease in mitochondrial proteins in diabetic animals, whereas EPI treated animals maintained levels close to sham animals. Further work will be done to evaluate mitochondrial KATP channel function and UCP expression. We expect that diabetic animals will have increased mitochondrial KATP channel dysfunction and increased UCP expression representing an overall drop in mitochondrial function. These results will provide evidence for the co‐occurrence of mitochondrial KATP channel dysfunction and UCP overexpression along with a possible treatment to prevent the progression of T2D.