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Antibiotic‐Induced Gut Microbiota Alters Period‐2 Circadian Gene Expression in Liver
Author(s) -
Leone Vanessa A,
Huang Edmond,
Wang Yunwei,
Devkota Suzanne,
Zale Elizabeth,
Dalal Sushila,
Chang Eugene B
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1205.2
Subject(s) - per1 , circadian rhythm , per2 , biology , gene expression , period (music) , gut flora , circadian clock , clock , endocrinology , gene , medicine , immunology , genetics , physics , acoustics
The circadian gene network in peripheral tissues has been shown to play a crucial role in driving and maintaining host metabolism. Studies suggest that gut microbiota also play a crucial role in host metabolism. A clear link between circadian gene network, host metabolism, and gut microbiota has yet to be elucidated. Our lab has shown circadian gene expression is altered in the absence of gut microbiota (germ‐free; GF). Liver samples from GF mice exhibited significant upregulation of circadian genes Cry2, Per1–3, and DBP, while Clock and Bmal‐1 expression were downregulated when compared to specific pathogen‐free mice (SPF). We then tested whether reducing gut microbes via antibiotic treatment alters circadian clock gene expression, similar to that seen in GF mice. SPF mice were exposed to antibiotic drinking water for 10 days and activity was monitored pre and post‐treatment. Mice were then harvested every 4 hours over a 24‐hour period and liver was analyzed for circadian gene expression. No differences in activity were exhibited between control and antibiotic‐treated animals. Only Per2 gene expression was significantly downregulated at t16:00 as compared to controls, resulting in a phase shift and reduced amplitude. While these results differ from those seen in GF mice, alterations in gut microbiota play a role in maintaining circadian gene expression in peripheral tissues.