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Isolation of adipose‐derived stem cells from human breast subcutaneous adipose tissues and their differentiation into beige adipose tissue‐like adipocytes
Author(s) -
Chen YuJen,
Kuo WenHong,
Yang JuiTing,
Chen ChihChien,
Ding ShihTorng
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1204.3
Subject(s) - adipose tissue , adipogenesis , stem cell , biology , medicine , endocrinology , stromal cell , white adipose tissue , microbiology and biotechnology
A new class of adipose tissue, termed beige adipose tissue (BeAT) has recently been identified to possess distinct origin, properties and function from the other two, white and brown adipose tissues, and implicated in regulating energy homeostasis that may affect metabolic syndrome. In an effort to study nutrient effects on the regulation of adipogenesis and amelioration of metabolic syndrome in humans, we identified BeAT‐like adipocytes differentiated from the human adipose‐derived stem cells (ADSC) isolated from the breast subcutaneous fats of breast cancer patients undergoing mastectomy. The procedure was approved by the Ethics Committee of National Taiwan University Hospital. The stromal‐vascular cells were isolated and maintained according to general procedures. After reaching to confluence and cultured in standard differentiation medium containing rosiglitazone for 15 days, the cells started to accumulated lipid droplets as stained by Oil‐Red‐O under light microscope and the expressions of brown adipose tissue‐or BeAT‐associated genes, such as UCP‐1, PRDM‐16, Tmem26, and CD137, were identified by quantitative real‐time PCR. Our current study, therefore, not only demonstrates that the human breast subcutaneous fat harbors the potential to differentiate into BeAT under adipogenic differentiation procedures, but also creates a novel model system for the study of BeAT and sheds new lights on future design of therapeutic approaches for metabolic diseases and regenerative medicine..