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MnTBAP Reduces Adiposity and Increases Bio‐markers for Mitochondrial Biogenesis
Author(s) -
Sosinsky Alexandra Z.,
Brestoff Jonathan R.,
Stansky Elena,
Fussell Leila,
Brodsky Timothy W.,
Donohue Jacob,
Sheppard Aaron,
Reynolds Thomas H.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1202.7
Subject(s) - mitochondrial biogenesis , ampk , medicine , endocrinology , amp activated protein kinase , phosphorylation , chemistry , lipolysis , thermogenin , adipose tissue , white adipose tissue , protein kinase a , biology , mitochondrion , biochemistry
The purpose of this study was to determine if manganese tetrakis benzoic acid porphyrin (MnTBAP) treatment reduces adiposity and increases bio‐markers for mitochondrial biogenesis. Male C57B6 mice were fed a low fat diet (LFD) or a high fat (HFD) for five months. During the last 30 days of the dietary intervention, mice were given intraperitoneal injections of MnTBAP (10 mg/kg/day) or vehicle. MnTBAP treatment significantly decreased body weight (52.8±2 vs. 40.1±2.1) and epididymal white adipose tissue (EWAT) weight (2.53±0.11 vs. 1.47±0.13). PGC‐1α and nuclear respiratory factor‐1 (NRF1) were significantly higher in EWAT from mice treated with MnTBAP compared to mice treated with vehicle. Additionally, MnTBAP treatment increased the phosphorylation of AMPK on Thr 172, site whose phosphorylation is associated with increased AMPK activity. Because β‐adrenergic receptor activation can increase AMPK activity and PGC‐1α expression, we also examined the phosphorylation of hormone sensitive lipase (HSL) on Ser 563, the site phosphorylated by the cAMP dependent kinase (PKA). Phosphorylation of HSL was significantly increased in EWAT of mice treated with MnTBAP, indicating the activation of β‐adrenergic receptor signaling. Taken together, our data demonstrates that MnTBAP treatment reduces dietary‐induced obesity by a mechanism that may involve mitochondrial biogenesis.