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Whole‐body heat stress increases mitochondrial enzyme activity in mice skeletal muscle
Author(s) -
Tamura Yuki,
Matsunaga Yutaka,
Masuda Hiroyuki,
Takahashi Yumiko,
Takahashi Yuki,
Takashima Satoshi,
Minagawa Hidehiro,
Hoshino Daisuke,
Hatta Hideo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1202.6
Subject(s) - ampk , skeletal muscle , medicine , endocrinology , protein kinase a , camk , mitochondrial biogenesis , p38 mitogen activated protein kinases , amp activated protein kinase , phosphorylation , citrate synthase , kinase , mapk/erk pathway , enzyme , chemistry , biology , mitochondrion , biochemistry , autophosphorylation
PURPOSE The purposes of present study were to investigate i) the chronic effects of whole‐body heat stress (WHS) on adaptation of mitochondrial enzyme activity and ii) the acute effects of WHS on kinase phosphorylation known to mediate mitochondrial biogenesis in mice skeletal muscle. METHODS ICR mice were divided into sedentary or WHS group. Mice in WHS group were exposed to hot environment chamber (30 × 20 × 13 cm, ambient temperature: 40 °C) for 30 min on each experiment day. In chronic experiment, we examined the effects of 3 weeks (Frequency: 5 days/week) WHS on maximal citrate synthase (CS) and 3‐Hydroxyacyl‐CoA dehydrogenase (3‐HAD) activities in plantaris muscle. In acute experiment, we studied the effects of a single WHS on phosphorylation of AMP activated protein kinase (AMPK), p38 mitrogen activated protein kinase (p38 MAPK) and calcium/calmodulin‐dependent protein kinase II (CaMK II) in plantaris muscle. RESULTS In chronic experiment, both maximal CS and 3‐HAD activities were increased in WHS group (CS: +35%, P<0.01, 3‐HAD: +49%, P<0.01). In acute experiment, phosphorylation of AMPK in WHS group was decreased (−43%, P<0.05). Phosphorylation of p38 MAPK was increased in WHS group (+252%, P<0.05). Phosphorylation of CaMK II was not different between groups. CONCLUSION Chronic whole‐body heat stress increased mitochondrial enzyme activities.