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Preservation of skeletal muscle by the heat shock proteins following frostbite injury
Author(s) -
Mestril Ruben,
Schweigert Colin,
Batey Jason,
Liskutin Tomas,
Baldwin John
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1200.1
Subject(s) - frostbite , skeletal muscle , heat shock protein , hindlimb , medicine , inflammation , oxidative stress , pathology , surgery , chemistry , biochemistry , gene
Background Skeletal muscle damage due to frostbite represents a serious medical problem. Oxidative stress and inflammatory response cause muscle degeneration following skeletal muscle injury. Studies have shown that the heat shock proteins (hsp) are able to protect against oxidative stress and inflammation. Results In the present study we used rat hindlimbs to examine the effects of several heat shock protein inducers, such as radicicol, 17‐AAG and 17‐DMAG following frostbite injury. Our results show that rat hindlimb muscles injected with these hsp inducers following frostbite injury exhibit less damage, less hypercontracted muscle fibers and less inflammatory cell infiltration as compared to control rat hindlimb muscles. We also find that these heat shock protein inducing compounds are able to conserve muscle function even when administered after frostbite injury has occurred. These results suggest that hsp inducing compounds are able to protect skeletal muscle if administered in the early stages following frostbite injury. Conclusion We conclude that compounds that induce heat shock proteins are able to preserve skeletal muscle function and structure if injected shortly after frostbite injury. Our studies provide the basis for the development of therapeutic strategies to treat injury caused by frostbite involving severe muscle damage. This research was supported by an award from the USAMRMC.