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The role of valproic acid (VPA) in regulating ischemia‐induced plasma extravasation into the peritoneum: implications for abdominal adhesion formation
Author(s) -
Brady Matthew,
King Elizabeth,
Heydrick Stanley,
Stucchi Arthur F
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1195.2
Subject(s) - extravasation , medicine , peritoneum , evans blue , ischemia , peritoneal cavity , saline , fibrin , laparotomy , adhesion , abdomen , anesthesia , pathology , surgery , chemistry , immunology , organic chemistry
Postoperative adhesions are a significant healthcare problem, occurring in over 94% of patients following abdominopelvic operations. Our lab showed that VPA reduces adhesion formation in a rat ischemic peritoneal button model; however its mechanism of action remains unknown. We proposed that VPA may blunt peritoneal plasma extravasation and reduce fibrin, the precursor of adhesions. This study aimed to create a method to quantify extravasation to test this hypothesis. Rats (n=11) underwent laparotomy and ischemic button creation. Animals received either intraperitoneal saline or VPA (50mg/kg) intraoperatively, followed by Evans Blue (25mg/kg) via tail vein injection. At 24 hr, plasma, ischemic button, and peritoneal tissue were collected. Evans Blue was extracted from tissue and its content was quantified by spectrophotometry. Plasma extravasation was increased in ischemic buttons compared with non‐ischemic inflamed peritoneal tissue in both groups (1.399uL/mg ±0 .086 vs. 0.288uL/mg ± 0.079, p<0.001) validating the model; however, VPA did not reduce plasma extravasation into peritoneal sidewalls, ischemic buttons, or peritoneal fluid when compared with control. Our data demonstrates that ischemia‐induced extravasation of plasma contents appears to be the primary source of peritoneal fibrin. Funded by Cooper‐Tyler Endowment, Department of Surgery, Boston University Medical Center.