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Perivascular adipose tissue contributes to large elastic artery stiffness with aging and is associated with greater superoxide bioavailability
Author(s) -
Fleenor Bradley Stephen,
Eng Jason S,
Pham Bryant T,
Kloor Jackson D,
Seals Douglas R
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1194.4
Subject(s) - adipose tissue , superoxide , pulse wave velocity , arterial stiffness , endocrinology , medicine , chemistry , artery , aorta , cardiology , bioavailability , blood pressure , pharmacology , biochemistry , enzyme
We tested the hypothesis that perivascular adipose tissue (PVAT) contributes to age‐related large elastic artery stiffening and is associated with greater superoxide production. PVAT from young (Y, 6 mo, n=5) and old (O, 27 mo, n=5) donor C57BL6/N mice was transplanted directly onto the abdominal aorta of Y recipient (6 mo) mice for 8 weeks. Y recipient mice transplanted with PVAT from O compared with Y donors had greater arterial stiffness as assessed by aortic pulse wave velocity (409 ± 7 vs. 342 ± 8 cm/s, P < 0.05) and ex vivo intrinsic mechanical stiffness (3197 ± 647 vs. 1889 ± 520 kPa, P < 0.05). Systolic (109 ± 5 vs. 111 ± 2 mmHg), diastolic (75 ± 5 vs. 82 ± 2 mmHg) and mean (86 ± 5 vs. 91 ± 2 mmHg) blood pressure did not differ between groups (n=3–4). Additional aortic segments from Y and O mice with (+) or without (−) PVAT (n=5–6) were cultured for 72 hours followed by intrinsic mechanical testing. Aortic segments from O+PVAT (3947 ± 276 kPa, P <0.05) had greater stiffness compared with Y+PVAT, Y−PVAT and O‐PVAT (2437 ± 226, 1768 ± 132 and 2668 ± 404 kPa, respectively); O‐PVAT had greater mechanical stiffening than Y−PVAT (P < 0.05). PVAT superoxide production was greater in O vs. Y (13835 ± 2322 vs. 6657 ± 1720 AU, P < 0.05, n=5–6). Collectively, these data demonstrate PVAT contributes to large artery stiffening with aging and is associated with increased superoxide bioavailability.