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The Effects of Palmitic Acid and Oleic Acid on Macrophage Gene Expression
Author(s) -
Reyes Ariel Delos,
Camell Christina,
Smith C. Wayne,
Rumbaut Rolando E.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1192.6
Subject(s) - palmitic acid , cd11c , oleic acid , macrophage , inflammation , gene expression , fatty acid , tumor necrosis factor alpha , biochemistry , chemistry , biology , gene , microbiology and biotechnology , immunology , in vitro , phenotype
Macrophages are critical in regulating inflammation and can be polarized to promote or inhibit inflammation. They have crucial roles in the clearance of excess fatty‐acids; however, their response to individual fatty‐acids are unclear. We hypothesized that bone marrow derived macrophages (BMDMs) treated with oleic acid will have increased anti‐inflammatory gene expression, and BMDM treated with palmitic acid will have increased pro‐inflammatory gene expression. We tested this hypothesis by culturing murine BMDM in the presence of oleic or palmitic acid and analyzing gene expression of markers for pro‐inflammatory (TNFα, CD11c and iNOS) and anti‐inflammatory macrophages (Arg1, MGL1, CD206, Chi3L3 and RETNLA). Palmitic acid increased TNFα expression though decreased CD11c and iNOS expression, whereas oleic acid increased Arg1 and MGL1 expression in BMDMs. We found no change in other genes examined. Additional macrophage markers and samples would need to be analyzed to clearly identify if fatty‐acids can polarize BMDMs; however, our data suggests that individual fatty‐acids can regulate expression of some pro‐ and anti‐inflammatory macrophage markers. Support: APS Frontiers in Physiology Fellowship; NRSA F‐31‐ DK084841 ‐04; USDA/ARS CHRC 6250–51000‐055