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Garlic (Allium sativum) attenuate glomerular glycation in Streptozotocin‐induced diabetic rats
Author(s) -
AlQattan Khaled K,
Thomson Martha,
Ali Muslim,
Mansour Mohamed H
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1192.5
Subject(s) - glycation , creatinine , medicine , endocrinology , streptozotocin , renal function , diabetes mellitus , allium sativum , urine , diabetic nephropathy , insulin , chemistry , biology , horticulture
Background In diabetes, hyperglycemia causes microangiopathy while garlic is effective in keeping euglycemia. Objective This study investigated the effect of 8 week‐single daily i.p. garlic extract injection on glomerular glycation in Streptozotocin‐induced diabetic rats. Method Urine volume and creatinine and serum insulin, glucose, creatinine and erythrocyte hemoglobin glycation (GHb) were measured at different times in diabetic‐control (D‐C), diabetic‐garlic (D‐G) treated and normal‐control (N‐C) rats. Images, glycation and count of normal and abnormal renal corpuscles (RC) were taken and estimated by light microscopy and Periodic Acid Shiff (PAS) staining. Results Compared to N‐C, D‐C showed significant decrease in serum insulin and increase in urine volume and creatinine and serum glucose, creatinine and GHb. D‐C also showed prevalent characteristic RC damage and intense diffused glomerular PAS activity. D‐G showed significant increase in serum insulin and decrease in urine volume and creatinine and serum glucose, creatinine, GHb. In D‐G significantly less damage of RC was obvious. In addition, PAS activity and distribution was close to that in N‐C. Conclusion Garlic is effective in inducing euglycemia ‐ one way by insulinemia ‐ thus attenuating glomerular glycation and preserving some renal clearance function.