Premium
c9,t11‐Conjugated linoleic acid alters HDL functionality
Author(s) -
Nicod Nathalie,
Parker Parker Stanley,
Giordano Elena,
Dávalos Alberto,
Visioli Francesco
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1192.4
Subject(s) - conjugated linoleic acid , chemistry , linoleic acid , basal (medicine) , conjugated system , cholesterol , biochemistry , fatty acid , endocrinology , organic chemistry , biology , insulin , polymer
Conjugated linoleic acid (CLA) is known to modulate plasma HDL levels. Here we report the effect of CLA on HDL functionality. Caco‐2 monolayers were grown on transwells, on which they can synthesize HDL and take up HDL‐cholesterol via basal SR‐BI. Cells were apically administered linoleic acid or different isomers of CLA (t9,t11‐CLA; c9,t11‐CLA; t10,c12‐CLA) in mixed micelles. HDL was secreted in the basal chamber for 24h in the absence or presence of an antibody to SR‐BI (aSR‐BI) to inhibit its interaction with HDL. Free (FC) and esterified cholesterol (EC) were determined in the basal chamber. FC in the presence of aSR‐BI was similar for all three isomers of CLA, but in the absence of aSR‐BI, FC accumulated significantly more with c9,t11‐CLA, suggesting a lack of uptake: 13% of FC was taken up via SR‐BI with c9,t11‐CLA vs. 30% with the other isomers. When the formation of pre‐β‐HDL particles was induced, cells treated with c9,t11‐CLA showed no uptake of FC via SR‐BI vs. a 25% uptake of FC with the other isomers. To determine if this effect was due to an alteration in HDL functionality, we added mature HDL to the basal chamber. c9,t11‐CLA induced a greater uptake of FC and EC via SR‐BI compared with the other isomers. Our results show that c9,t11‐CLA treatment induces the formation of a less functional pre‐β‐HDL particle. On the other hand, c9,t11‐CLA stimulates a more efficient uptake of cholesterol from a mature HDL particle.