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Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance
Author(s) -
HE GuoWei,
GAO Ge,
XUAN Chao,
BAI XiaoYan,
LIU XiaoCheng,
JING WenBin,
YANG Qin
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1189.3
Subject(s) - vitronectin , transferrin , medicine , carbonic anhydrase , proteomics , c4a , alpha (finance) , blood proteins , haptoglobin , endocrinology , chemistry , biology , microbiology and biotechnology , biochemistry , enzyme , gene , receptor , surgery , integrin , construct validity , patient satisfaction
The genetic basis and proteomics of valvular heart disease (VHD) are important for diagnosis and treatment but little is known about biomarkers in rheumatic (RVD) and degenerative (DVD) VHD. Differential plasma protein analysis identified 18 differentially expressed protein sports and 14 corresponding proteins or polypeptides by two‐dimensional electrophoresis and mass spectrometry in 120 subjects. Two up‐regulated (complement C4A and carbonic anhydrase 1) and three down‐regulated proteins (serotransferrin, alpha‐1‐antichymotrypsin, and vitronectin) were validated by ELISA in enlarging samples. The plasma levels (n=40 in each group) of complement C4A in RVD (715.8±35.6 vs. 594.7±28.2 ng/ml, P=0.009) and carbonic anhydrase 1 (237.70±15.7 vs. 184.7±10.8 U/L, P=0.007) in DVD patients were significantly higher and that of serotransferrin (2.36±0.20 vs. 2.93±0.16 mg/ml, P=0.025) and alpha‐1‐antichymotrypsin (370.0±13.7 vs. 413.0±11.6 μg/ml, P=0.019) in RVD patients were significantly lower than those in controls. The plasma vitronectin in both RVD (P=0.006) and DVD (P=0.01) groups was significantly lower than those in normal controls. These revealed potentially useful biomarkers and possible genetic deficiency in valvular diseases. Supported by NSF 81170148, 2010CB529500, 2009DFB30560, 09ZCZDSF04200, 10JCYBJC26400, 2012‐BH110004, 2011BHKZ001 & 2011BHKY002, and CUHK4789/09, China.