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Study of Zebrafish Cardiac Morphogenesis Using Computational Fluid Dynamics
Author(s) -
Lee Juhyun,
Cao Hung,
Hsiai Tzung Keith
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1187.8
Subject(s) - intracardiac injection , zebrafish , shear stress , morphogenesis , computational fluid dynamics , atrioventricular valve , biomedical engineering , anatomy , cardiology , mechanics , medicine , chemistry , physics , biochemistry , ventricle , gene
Hemodynamics is intimately linked to cardiac structural morphogenesis. Intracardiac blood flow creates shear stresses and pressure forces on the endoluminal surface of developing heart tubes. Zebrafish is a genetically tractable model to investigate the cardiac morphogenesis owing to their transparency and fast cardiac development in embryo stages. However, in vivo and real‐time measurement of intracardiac shears stress and pressure in zebrafish is challenging due to size limitation. Computational Fluid Dynamics (CFD) model with specific boundary conditions [may provide an entry point to establish the indirect measurement of intracardiac parameters over time. Here, we quantified intracardiac shear stress and pressure starting from 30 hours post fertilization (hpf) to 120 hpf. To reconstruct 2‐D dynamic boundary conditions, CFD simulation was used to demonstrate incremental increase in shear stress along the atrioventricular (AV) valve region. The decrease in the diameter of AV valve was consistent with the increase in blood velocity. At 120 hpf, AV valve was fully developed to allow for comparing changes in intracardiac shear stress. Our dynamic CFD modeling provides a basis to elucidate the molecular mechanisms whereby biomechanical forces modulate the AV valvular morphogenesis.