Premium
Neonatal Phthalate Exposure Induces Cardiac Dysfunction in Adolescent Rats
Author(s) -
Yang ShuHan,
Yang ReiCheng,
Wang DeanChuan
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1184.8
Subject(s) - phthalate , ventricle , medicine , bradycardia , shock (circulatory) , physiology , hsp70 , endocrinology , heat shock protein , chemistry , heart rate , biochemistry , blood pressure , gene , organic chemistry
Di‐(2‐ethylhexyl) phthalate (DEHP) is a widely used plasticizer found in a variety of polyvinyl chloride (PVC) products. Many evidences suggest that DEHP leached from PVC products may cause health problems in humans. Treatment of DEHP leads to arrhythmia in cultured cardiomyocytes suggesting that DEHP may act as an arrhythmogenic agent. Because infant babies and school‐age children are more vulnerable to environmentally toxic agents, there is a need to evaluate the effects of neonatal DEHP exposure‐induced cardiac dysfunction in adolescent rats. Sprague‐Dawley dams were orally fed with DEHP (10 mg/kg/day) or corn oil everyday during lactational period. At the age of 8 weeks, male pups were anesthetized and subjected to electrocardiogram (ECG) detection and then sacrificed for heart isolation. The proteins from left ventricle were extracted for Western blotting. The results show that bradycardia and prolonged QT intervals were observed in DEHP group. Meanwhile, the expressions of heat shock protein 70 (HSP70), a biomarker of protective mechanism in myocardium, were significantly decreased in DEHP group. These results indicate that DEHP exposure in the early life stage may cause cardiac dysfunction by decreased HSP70 protection in adolescent animals.