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Developing methods to enhancing cell engraftment in a genetically engineered mouse model
Author(s) -
Hsu Yu Chen,
Kao ChungYang,
Tsai MingShian,
Yu IShing,
Chen ChunYu,
Lin ChienYu,
Lee FangJen,
Wu YaoMing,
Lin ShuWha
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1181.4
Subject(s) - stem cell , cell , cell culture , cell therapy , genetically modified organism , transplantation , cancer research , biology , microbiology and biotechnology , immunology , medicine , genetics , gene
Cell/stem cell transplantation would be a promising approach for the treatment of genetic liver diseases and end‐stage liver diseases. Since a significant hurdle is the poor engraftment efficiency in the host liver, developing methods to enhance cell engraftment is important for cell/stem cell therapy. We have generated a genetically engineered mouse (GEM) line with narrowed sinusoidal diameters, which is also associated with the cancer cells preferentially colonized in the mouse liver as a result of retention of tumor cells. In the current study, we used the primary hepatocytes from R26R‐GR mice, a double‐fluorescent‐reporter knockin mouse line that could be used as a reporter system for investigation of cell therapy conditioning. We shown the donor cell engraftment can be improved in this GEM line. After the equivalent number of the primary hepatocytes was transplanted, the efficiency of cell engraftment was significantly greater in the GEM than that in WT mice at all the time points. We demonstrated that the R26R‐GR mouse could be a good tool to trace the donor cells in the research for cell/stem cell therapy, and that the donor cells could enhance engraftment in our GEM line. We suggest that this strategy may be applicable to improve cell/stem cell therapy in the near future.