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MALT Lymphoma Stem Cell and its Niche in Helicobacter heilmannii‐infected Mice Stomach
Author(s) -
Nakamura Masahiko,
Matsui Hidenori,
Takahashi Tetsufumi,
Tsuchimoto Kanji,
Takahashi Shinichi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1181.1
Subject(s) - biology , helicobacter , malt lymphoma , stem cell , pathology , helicobacter pylori , lymphoma , gastric lymphoma , cd5 , stem cell marker , immunology , medicine , genetics
Background & Aims Although cancer and leukemia stem cells are indispensable in the initiation and enlargement of lesions, the gastric MALT lymphoma is thought to be polyclonal with the persistent infection of Helicobacter species and then change to monoclonal with genetic aberrations, and the participation of stem cells in the tumor formation remains to be elucidated. We performed a histochemical analysis in Helicobacter heilmannii‐ induced gastric and hepatic MALT lymphoma in the infected alone and eradicated after infection groups. Materials and Methods We used a Helicobacter heilmannii sample isolated from the stomach of a cynomolgus monkey and maintained in C57BL/6 mouse stomachs. Mucosal homogenates were used to inoculate C57BL/6 mice, which were then examined over 24 months. Macroscopic observations were carried out, and PCR analysis of the bacteria of the Helicobacter species was performed at intervals over the observation period. Histochemical analysis was performed using the gastric and hepatic MALT lymphoma with monoclonal and polyclonal stem cell‐related antibodies against doublecortin‐like kinase (DCAMKL1), Musashi‐1, proliferating cell nuclear antigen (PCNA), CD44, CD133, as well as myofibroblast, endothelial, and pericyte markers. Results DCAMKL1, Musashi‐1, CD144 positivities were not recognized in the early stage of the lymphoma formation but gradually found in the lymphocytes located in the marginal zone of the MALT lymphoma both in the gastric and hepatic MALT lymphoma. The well‐developed microcirculatory network accompanied the stem cell rich area. The myofibroblasts were also distributed in these area, suggesting the formation of stem cell niche similar to that found in epithelial cells. Conclusions MALT lymphoma stem cells were found to exist in the marginal zone of the lymphoma, constituting the niche by the myofibroblast and microcirculatory components.