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Effects of Parabolic Flight on Serotonin‐related Gene Expression in the Mouse Brain
Author(s) -
Yoshioka Mitsuhiro,
Yamaguchi Taku,
Ohta Hisashi,
Ochiai Toshimasa
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1176.4
Subject(s) - serotonergic , tryptophan hydroxylase , serotonin transporter , serotonin , endocrinology , midbrain , medicine , gene expression , tyrosine hydroxylase , tryptophan , 5 ht receptor , biology , receptor , gene , central nervous system , biochemistry , dopamine , amino acid
Exposure to uncontrollable stress triggers a wide range of adaptive changes in the CNS including the elevation of serotonin metabolism and an increased susceptibility to affective disorders. The purpose of the present experiment is to elucidate whether gene expression levels, especially serotonin‐related genes, are altered in the mouse brain exposed to gravity‐changing stress using a competitive reverse transcription‐polymerase chain reaction. Mice were exposed to gravity‐changing stress during 8 times repeated by parabolic flight. Serotonin and tryptophan transporter, and tryptophan hydroxylase‐2 mRNA levels in the midbrain 6 h after the flight were significantly increased compared with pre‐parabolic flight control. In contrast, those of 5‐HT1A receptor, GAD65/67, and tyrosine hydroxylase were not altered by the flight. These results suggest that the serotonergic system, particularly presynaptically synthetic pathway, might be activated in the CNS by gravity‐changing stress.