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Regulation of Potassium‐induced [ 3 H]D‐Aspartate Release from Isolated Bovine Retina by 5‐epi‐5‐F 3t ‐Isoprostane: Role of Prostanoid Receptors
Author(s) -
Opere Catherine A,
Jamil Jamal,
Salvi Ankita,
Bankhele Pratik,
Flowers Arnecia,
Floyd Namonique,
Durand Thierry,
Galano JeanMarie,
Guy Alexandre,
Oger Camille,
NjieMbye Ya Fatou,
Kulkarni Madhura,
Ohia Sunny E
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1174.6
Subject(s) - chemistry , prostanoid , receptor , neurotransmitter , arachidonic acid , medicine , endocrinology , biochemistry , biology , enzyme
Arachidonic acid‐derived F 2 ‐isoprostanes (F 2 ‐IsoPs) can regulate neurotransmitter release in bovine retina ( Jamil J. et al, 2012 ). It is, however, unclear whether eicosapentanoic acid‐derived F 3 ‐IsoPs can produce a similar effect in the retina. Purpose To investigate the pharmacological actions of 5‐ epi ‐5‐F 3t ‐IsoP on K + ‐induced [ 3 H]D‐aspartate release from bovine retina. We also examined the role of prostanoid receptors on the F 3 ‐IsoP‐induced response. Method Isolated neural retina were incubated in oxygenated Krebs solution containing 200 nM of [ 3 H]D‐aspartate and then prepared for studies of neurotransmitter release. Release of [ 3 H]D‐aspartate was evoked by K + (50 mM) stimuli applied at 90 mins (S 1 ) and at 108 mins (S 2 ) after the onset of superfusion. Results 5‐ epi ‐5‐F 3t ‐IsoP (0.1 nM – 0.1 μM) elicited an inhibitory action on K + ‐evoked [ 3 H]D‐aspartate release in a concentration‐dependent manner, achieving a maximum inhibition of 46.9% at 0.1 μM (IC 30 of 1 nM). The effect of 5‐ epi ‐5‐F 3t ‐IsoP (0.01 μM) was partially reversed by the prostanoid receptor antagonists, SC 19220 (1 μM; EP 1 ), SC 51322 (10 μM; EP 1 ) and AH 6809 (10 μM; EP 1–3 /DP 1 ) while AH 23848 (1 μM; EP 4 /TP 1 ) and SQ 29548 (10 μM; TP) had no effect on the IsoP. Conclusion 5‐ epi ‐5‐F 3t ‐IsoP can inhibit K + ‐evoked [ 3 H]D‐aspartate release in isolated bovine retina, presumably via mechanisms that involve prostanoid receptors.