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Aspirin influence on mice sex genders during cutaneous wound healing
Author(s) -
Santos Jeanine Salles,
Costa Andréa Monte Alto
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1168.8
Subject(s) - myeloperoxidase , aspirin , hydroxyproline , wound healing , medicine , von willebrand factor , vascular endothelial growth factor , lesion , endocrinology , andrology , pathology , inflammation , surgery , vegf receptors , platelet
The aim of this study was to evaluate whether aspirin, and thus cyclooxygensase (COX) inhibition, affects cutaneous wound healing in distinctly female and male mice. Female and male Balb/c mice were treated with aspirin (25 mg/kg/day) until euthanasia. The control group received vehicle only. A full‐thickness excisional lesion was performed, and macroscopic, histological and biochemical parameters were evaluated. Sections were stained and immunostained for microscopic analysis. Myeloperoxidase activity, hydroxyproline quantity and protein expression of von Willebrand factor (vWF) and vascular endothelial growth factor (VEGF) were also measured. Female control and treated groups presented delayed contraction and reepithelialization when compared to male control and treated group, respectively. Female control group presented reduced myeloperoxidase activity and MIF‐positive cells compared to male control group. In female treated group, myeloperoxidase activity and F4/80‐positive macrophages quantity was greater than in control group. Collagen was reduced only in female treated group. vWF and VEGF protein expression were increased in female‐treated group. In summary, aspirin administration impaired wound healing process in Balb/c females but not in males.