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Wound healing activity of (−)‐borneol complexed to chitosan bioactive film in rodents
Author(s) -
Souza Siqueira Barreto Rosana,
Silva Prado Douglas,
Galvão Juliana Gouveia,
Cunha Gonsalves Joyce Kelly Marinheiro,
Souza Nunes Rogéria,
AlbuquerqueJúnior Ricardo Luiz Cavalcanti,
Soares Rosilene Calazans,
Lucca Waldecy,
QuintansJunior Lucindo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1168.2
Subject(s) - borneol , chitosan , wound healing , granulation tissue , chemistry , pharmacology , medicine , biochemistry , pathology , surgery , alternative medicine , traditional chinese medicine
The aim of this study was to investigate the wound healing activity of (−)‐borneol complexed to chitosan film on excision wound model in rats. Excision wound: Sixty rats, Wistar , 250–300 g were anesthetized and an excisional wound was performed. The animals was treated with placebo (inert chitosan bioactive film), (−)‐borneol 0.5% and (−)‐borneol 1%, for 3 and 7 days. The residual wounds were analyzed and removed. The activity of the enzyme myeloperoxidase was analyzed at 3 days. Serial histological sections were obtained and stained by histochemical techniques (Hematoxilin‐Eosin – HE and Sirius red) and immunohistochemical techniques. Experimental protocols were approved by the Animal Care and Use Committee at the Federal University of Sergipe (CEPA#01/2011). The results suggest that the chitosan bioactive film with (−)‐borneol reduced (p < 0.05) wound areas, induced earlier granulation tissue formation and increased cell proliferation when compared to the control group. And the chitosan bioactive film with (−)‐borneol 0.5% and (−)‐ borneol 1% decreased (p < 0.05) the activity of the enzyme myeloperoxidase. It is suggested that (−)‐borneol modulates the wound healing process.