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Antibacterial activity of methanolic extracts from Dunaliella salina and Chlorella vulgaris
Author(s) -
MedinaJaritz Nora B.,
CarmonaUgalde Luis Fernando,
LopezCedillo Julio Cesar,
Leon F. Sandra L. RuilobaDe
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1167.5
Subject(s) - proteus vulgaris , chlorella vulgaris , dunaliella salina , bacillus subtilis , staphylococcus aureus , salmonella typhi , antibacterial activity , microbiology and biotechnology , agar diffusion test , microorganism , food science , biology , agar , chemistry , escherichia coli , algae , bacteria , botany , biochemistry , genetics , gene
Many antibiotic substances have been isolated from algae, but little from marine green microalgae like Dunaliella salina. To examine the antibiotic effect of extracts from Dunaliella salina and Chlorella vulgaris, methanolic extracts were tested against Proteus vulgaris, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Salmonella typhi ATCC 6534, E. coli ATCC 8739, S. aureus ATCC 25923 and B. subtilis ATCC 6635. Algae biomass were subjected to methanol/acetic acid treatments, dried and resuspended in methanol. The microorganisms were grown in a Mueller Hinton culture media; for the agar diffusion test were adjusted to tube 1 nephelometer and spread on plates; extracts were impregnated onto paper discs. After incubation inhibition zones around each disc were measured. One‐way ANOVA Dunnett analysis was performed. The D. salina extract showed significant antibacterial activity against all microorganisms, especially to Proteus vulgaris and Salmonella typhi ATCC 6534; while the C. vulgaris extract did not show significant antibacterial activity against all microorganisms, but the bigger inhibition halos result with B. subtilis and B. subtilis ATCC 6635. Support from SIP‐IPN and COFAA‐IPN.