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Complementary roles of KCa3.1 channels and β1‐integrin in the regulation of alveolar epithelial repair
Author(s) -
GIRAULT alban,
BASTIEN Rachel,
TRINH Nguyen Thu Ngan,
PRIVE Anik,
MAILLE Emillie,
BROCHIERO Emmanuelle
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1166.3
Subject(s) - fibronectin , integrin , extracellular matrix , microbiology and biotechnology , wound healing , alveolar epithelium , chemistry , resorption , regeneration (biology) , matrix (chemical analysis) , pathology , epithelium , medicine , immunology , cell , biology , biochemistry , chromatography
A common feature of acute lung injury is extensive damage and remodeling of alveolar epithelium. It has been shown that epithelial regeneration and secondary lung edema resorption are crucial to patient recovery. We previously reported that KvLQT1 and K ATP K + channels regulated alveolar epithelial repair processes, but the role of another candidate, the KCa3.1, has never been evaluated. We first demonstrated that the wound‐healing rates were higher in primary rat alveolar cell monolayers cultured on a fibronectin‐collagen (FC) matrix, than on non‐coated supports. KCa3.1 inhibition, with TRAM‐34, or activation, with 1‐EBIO, respectively down‐ and up‐regulated the alveolar wound‐healing rates on FC matrix, but were without effect in absence of FC coating. Accordingly, single cell migration rates were also dependent, at least in part, of FC matrix coating and KCa3.1 activity. A possible relationship between KCa3.1 and migratory cell proteins such as the integrin, activated by fibronectin, was then assessed. Co‐immunoprecipitation experiments thus revealed a physical interaction between KCa3.1 and the β‐1 integrin subunit. Altogether, our data showed for the first time a complementary role of KCa3.1 channels, extracellular matrix and β‐1 integrin, in the regulation of alveolar repair after injury. Project funded by CIHR, RHN of FRQS, and FESP of Université de Montréal.