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Resveratrol relaxes cholecystokinin‐ and KCl‐induced tension in guinea pig gallbladder strips by inhibiting extracellular Ca 2+ entry
Author(s) -
Kline Loren W,
Karpinski Edward
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1157.1
Subject(s) - chemistry , cholecystokinin , chelerythrine , resveratrol , extracellular , relaxation (psychology) , genistein , egta , endocrinology , medicine , biochemistry , calcium , protein kinase c , organic chemistry , receptor , enzyme
Resveratrol (Res) belongs to a class of polypheloic compounds called stilbenes and is a phytoalexin. Res relaxed cholecystokinin octapeptide (CCK) or KCl‐induced tension. The relaxation was concentration dependent. An in vitro technique was used. Paired t‐tests were used. Differences between mean values with p<0.05 were considered significant. To determine if the PKA/cAMP second messenger system mediated the Res‐induced relaxation, PKA inhibitor 14–22 amide myristolated (PKA‐IM; 180 nM) was used and had no significant effect on Res‐induced relaxation. Bisindolymaleide IV (0.5 μM) and chelerythrine Cl − (5 mM)were used together, a significant (p<0.05) increase in Res‐induced relaxation (61.8±3.5 vs. 73.7±5.6% was observed. 2‐APB (125 μM), significantly (p<0.01) decreased the amount of Res‐induced relaxation (59.7±3.6 vs. 45.9±5.6%). Genistein (10 μM) significantly (p<0.01) increased the Res‐induced relaxation (53.4±2.2 vs. 61.8±3.8%). Neither L‐NMMA (20μM), KT5823 (585 nM), nor fulvestrant (10 μM had a significant effect on the amount of Res‐induced relaxation. Adding Res (75μM) prior to the KCl significantly (p<0.001) decreased the amount of KCl‐induced tension (0.85±0.06 vs. 0.43±0.04 g). Since both CCK and KCl act via L‐type Ca 2+ channels and the amount of tension generated is significantly decreased with the prior addition of Res, Res likely exerts its effects via L‐type Ca 2+ channels.