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New experimental model of gastric carcinogenesis in rats with the use of formaldehyde and hydrogen peroxide mixture
Author(s) -
Senchukova Marina,
Stadnikov Alexsander A,
Bokov Dmitry A
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1156.2
Subject(s) - lymph , stomach , pathology , lamina propria , adenocarcinoma , gastroenterology , lymphatic system , gastric mucosa , medicine , metastasis , hydrogen peroxide , cancer , chemistry , epithelium , organic chemistry
This study was designed to develop an animal model of gastric cancer by using a mixture of formaldehyde and hydrogen peroxide. Thirty‐five outbred rats were used. In experimental group, 1.0 ml of the formaldehyde (0.4%) and hydrogen peroxide (0.4%) mixture (1:1) were administered to 30 rats by intragastric gavage on the 1 st and 3 rd day every 2 weeks for 6 months. Five non‐treated rats served as a control. The mortality rate among treated rats was 13.3%. We found that 6 months after the onset of treatment the 4 of 5 rats had gastric tumor, while after 7.5 months all survived 21 rats showed malignant tumors of the stomach. The tumors were primarily localized within the gastric mucosa, though the invasion of individual tumor cells and their clusters in the muscularis propria and serosa as well as in the lymphatic and blood vessels were also seen. In most cases, the infiltrated gastric mucosa areas (2 to 3) had rigid gastric folds and were 0.7×1.0cm to 1.5×3.0cm in size. We observed signet‐ring cell carcinomas in 80.9% of rats, well‐differentiated tubular adenocarcinoma in 66.6% of rats and moderately differentiated tumors in 23.8% of rats. Metastasis in regional lymph nodes were noted in 3 rats (14.3 %), while 6 animals (28.6%) revealed peritoneal dissemination of the small intestine. In addition, 19.0% of rats showed tumors of the lungs. Therefore, these data demonstrate that our animal model can be used to study gastric carcinogenesis.