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Protective role of Selenium and Adiponectin deficiency in chronic inflammation induced colon cancer.
Author(s) -
SAXENA ARPIT,
KAUR KAMALJEET,
Fletcher Emma,
Larsen Bianca,
Hegde Shweta,
FAYAD RAJA
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1156.1
Subject(s) - adiponectin , colorectal cancer , inflammation , carcinogenesis , azoxymethane , cancer , medicine , gastroenterology , apoptosis , endocrinology , weight loss , selenium , immunology , chemistry , obesity , biochemistry , insulin resistance , organic chemistry
Selenium (Se), a nutrition supplement and a well known antioxidant, has been shown to inhibit tumorigenesis in several experimental cancer models. Adiponectin (APN), an adipocytokine secreted by the adipocytes has been shown to play a protective role in chronic inflammation induced colon cancer (CICC). Inflammation and colon cancer were induced in both APN knockout (KO) and C57B1/6 wild type (WT) mice by 2% dextran sodium sulfate (DSS) and 1,2‐Dimethylhydrazine (DMH) treatment respectively and were divided into 8 treatment groups (n=9 per group): 1) No treatment + normal diet (N); 2) Group 1 + Se; 3) treatment with three cycles of DSS + N; 4) Group 3 + Se; 5) weekly doses of DMH (20mg/kg of mouse body weight) for twelve weeks + N; 6) Group 5 + Se; 7) a single dose of DMH followed by 3 cycles of DSS + N; 8) a Group 7 + Se. Mice were observed for diarrhea, stool hemoccult, and weight loss and were sacrificed on day 192. Tissues were collected from the colon for genomic and proteomic studies. Se aided in the reduction of the physical signs and the histopathological scoring associated with CICC with APNKO mice having worse score when compared to WT mice. Se concentration, Gpx activity, Gpx protein expression and tumor cell apoptosis was found to be significantly higher in the colon of the mice given Se diet. Other proteomic and genomic studies also indicate the protective role of Se in the progression and the symptoms associated with CICC.