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TRPV1 in the paraventricular nucleus of the hypothalamus is involved in the regulation of systemic blood glucose levels
Author(s) -
Zsombok Andrea,
Miyata Kayoko,
O'Hare James D,
Fourrier Torrey L,
Krantz Amanda M,
Derbenev Andrei V
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1154.13
Subject(s) - medicine , endocrinology , trpv1 , hypothalamus , chemistry , insulin , glucose homeostasis , gluconeogenesis , arcuate nucleus , glycogen synthase , receptor , biology , insulin resistance , metabolism , transient receptor potential channel
The autonomic nervous system plays a pivotal role in the regulation of glucose homeostasis. Transient receptor potential vanilloid type 1 (TRPV1) receptors are expressed in the paraventricular nucleus (PVN) of hypothalamus and liver‐related PVN neurons are controlled by TRPV1‐dependent excitatory neurotransmitter release. However, the effect of TRPV1 activation on whole body glucose homeostasis remains to be determined. Capsaicin (0.5 nmol), a TRPV1 agonist was injected bilaterally into the PVN and systemic blood glucose levels, serum insulin levels, expression of proteins involved in hepatic gluconeogenesis and in glucose uptake in the skeletal muscle were measured in male CD1 mice. Activation of TRPV1 in the PVN significantly decreased systemic blood glucose levels. The reduction was detectable up to 30 min, with a maximum approximately at 15 min. The glucose lowering effect of TRPV1 was suppressed in the presence of the glutamate receptor antagonist, kynurenic acid. Serum insulin levels did not show significant difference between groups. Activation of TRPV1 in the PVN decreased hepatic PEPCK and pyruvate carboxylase expression levels and increased phosphorylation of glycogen synthase kinase 3 in the skeletal muscle. Our data suggest that TRPV1 in the PVN modulates systemic blood glucose levels. Supported by Tulane BIRCWH 2K12HD043451, Tulane Aging CoBRE P20GM103629.