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Impaired skeletal muscle metabolism in obesity prone rats on a high fat diet
Author(s) -
Allerton Timothy Daniel,
Bench Eli,
Primeaux Stefany
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1153.4
Subject(s) - glut4 , endocrinology , medicine , skeletal muscle , cd36 , lipid metabolism , glucose transporter , insulin resistance , carbohydrate metabolism , gastrocnemius muscle , biology , peroxisome proliferator activated receptor , western blot , chemistry , insulin , biochemistry , receptor , gene
CD36 and Glut4 are major transporters of fatty acids and glucose and PPARγ is an important modulator of insulin sensitivity and lipid sensing in skeletal muscle, especially when consuming a high fat diet (HFD). Dysregulation of these transporters and modulators in skeletal muscle may influence the susceptibility to obesity. The purpose of the current experiment was to determine the effects of a HFD on factors associated with glucose and lipid storage and metabolism in the skeletal muscle of obesity‐prone and obesity‐resistant rats. Osborne‐Mendel (OM) and S5B/Pl (S5B) rats were fed a HFD or a low fat diet for 2 weeks. The gastrocnemius muscle was removed and tissue was sliced and stained for oxidative enzymes or protein was isolated for determination of protein expression of CD36, Glut4, and PPARγ using Western Blot. OM rats had a higher proportion of oxidative muscle fibers compared to S5B rats. Additionally, OM rats had a greater expression of CD36 compared to S5B rats, which was independent of diet. The consumption of a HFD reduced Glut4 levels in the skeletal muscle of OM rats compared to S5B rats and OM rats expressed less PPARγ than S5B rats, regardless of diet. Overall, these differences suggest that OM rats consuming a HFD have impaired expression of the main glucose transporter and a key modulator of lipid detection, PPARγ, in skeletal muscle, which may contribute to their susceptibility to obesity.