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Old age of male C57/BL6 mice coincides with development of sarcopenia and anabolic resistance
Author(s) -
Dijk Miriam,
Nagel Jolanda,
Dijk Francina J,
Walrand Stephane,
Norren Klaske,
Luiking Yvette
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1150.6
Subject(s) - sarcopenia , anabolism , medicine , skeletal muscle , endocrinology , muscle mass , ageing , grip strength , physiology
Ageing is associated with sarcopenia, a progressive decline of skeletal muscle mass, muscle quality and muscle function. Although muscle parameters have been studied in mice of various ages/strains, no single study characterized these parameters in mice of various ages of the same strain. Therefore, male 10 and 24 mo old C57BL6/6J mice (n=45/group) were studied for the parameters of sarcopenia and the presence of anabolic resistance as part of its pathophysiology, with the aim of characterizing sarcopenia in old mice. Statistics were performed by ANOVA with post hoc LSD. Muscle mass of the hind limb was lower in 24mo (426±10mg) compared to 10mo (481±8mg) old mice (P<0.05). A significant decrease in physical daily activity (−62.8%), muscle grip strength (−11.1%) and ex vivo muscle contractile function (−22.4% maximal force production) was observed in 24mo compared to 10mo old mice (P<0.05). The muscle anabolic response to a single standard meal was tested and showed increased muscle protein synthesis in young, but not in old mice (P<0.05) indicative of anabolic resistance in the old mice. This study shows clear characteristics of sarcopenia and anabolic resistance in 24mo old C57/BL6 male mice. These observations offer potential to explore the effects of interventions targeting sarcopenia in the future using this mouse model.