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Inhibition of NFκB activity in renal collecting duct cells abolishes transepithelial voltage difference
Author(s) -
Svenningsen Per,
Jensen Boye L
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1148.14
Subject(s) - chemistry , nf κb , homeostasis , cell culture , duct (anatomy) , kidney , sodium , medicine , endocrinology , biology , anatomy , signal transduction , biochemistry , organic chemistry , genetics
The renal collecting ducts are of major importance for regulation whole body sodium homeostasis and blood pressure. NFκB is a transcription factor which has been shown to influence transepithelial sodium transport in the renal collecting duct. The aim of the present study was to test the effect of NFκ inhibition on transepithelial voltage (TEV) and resistance (TER) in the mouse cortical collecting duct cell line M‐1. M‐1 cells were grown for 8 days with vehicle or 100 nM NFκB inhibitor on Snapwell supports, and TEV and TEV were measured daily. NFκB inhibition reduced the TEV significantly, which on day 8 reached 17.4 ± 1.5 mV in vehicle treated cells versus 0 ± 0 mV in inhibitor treated cells. In contrast, NFκB inhibition increased TER, which on day 8 reached 2118 ± 411 Ω in vehicle treated cells versus 3066 ± 208 Ω in inhibitor treated cells. It was observed that treatment of the cells with NFκB inhibition led to acidification of the apical and basolateral culture medium; thus, the pH in vehicle treated cells were 6.89±0.01 and 6.99±0.01, respectively, whereas pH of the inhibitor treated cells were 6.82±0.02 and 6.91±0.01, respectively. Thus, the current data suggest that NFκB activity is essential for development of a functional renal collecting duct.