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Osteoblast Differentiation of Adipose Tissue‐Derived Mesenchymal Stem Cells (MSCs) is Enhanced by Bone Marrow MSC‐Conditioned Medium
Author(s) -
Beloti Marcio Mateus,
Abuna Rodrigo Flores,
Oliveira Fabiola Singaretti,
Kato Rogerio Bentes,
Rosa Adalberto Luiz
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.1146.3
Subject(s) - mesenchymal stem cell , adipose tissue , bone sialoprotein , bone marrow , osteocalcin , osteoblast , stem cell transplantation for articular cartilage repair , paracrine signalling , microbiology and biotechnology , runx2 , chemistry , biology , immunology , cellular differentiation , endocrinology , adult stem cell , alkaline phosphatase , in vitro , biochemistry , receptor , gene , enzyme
Mesenchymal stem cells (MSCs) derived from several adult tissues may be driven to differentiate into distinct cell lineages, making them the focus of a plethora of studies on cell therapy and tissue engineering. Recently, we have noticed that adipose tissue‐derived MSCs exhibit a lower osteogenic potential compared with bone marrow MSCs. As MSCs secrete many growth factors that could affect cells in terms of genotype and phenotype expression, here, we investigated the effect of conditioned medium from bone marrow MSCs on osteoblast differentiation of adipose tissue‐derived MSCs. Rat bone marrow MSCs were cultured in osteogenic medium, which was collected every 48 hours during 14 days and stored at −80ºC. This conditioned medium was used to culture adipose tissue‐derived MSCs for periods of up to 17 days and non‐conditioned osteogenic medium was used as a control. Real‐time RT‐PCR for key bone markers was carried out in quintuplicate (n=5) and data were compared by Mann‐Whitney test (p<0.05). Conditioned medium upregulated gene expression of Runx2 and bone sialoprotein at 4 and 10 days, collagen, type I, alpha 1 at 10 and 17 days, and osteocalcin at 4, 10 and 17 days when compared with non‐conditioned medium. Our results indicate that bone marrow MSCs synthesize paracrine factors that favor osteoblast differentiation of adipose tissue‐derived MSCs. Thus, this approach is feasible for obtaining an osteoblast‐enriched cell population from adipose tissue‐derived MSCs to be employed in cell therapy and bone tissue engineering research.